Integrated Determination of Phenolic Compounds and Antioxidant Activity in Pineapple Peel (Ananas comosus L. Merr) via TLC-Densitometric Approach

Pineapple peel (Ananas comosus L. Merr), often regarded as waste and rarely utilized, possesses significant potential as a natural source of antioxidants. This study aims to evaluate the antioxidant activity and total phenolic content of pineapple peel fractions. The methods employed include extraction by refluxing with ethyl acetate as the solvent, followed by liquid–liquid fractionation. Antioxidant activity was assessed using the DPPH method with UV-Vis spectrophotometry, while total phenolic content was determined using the Folin–Ciocalteu reagent, also analyzed via UV-Vis spectrophotometry. Phytochemical profiling of the fractions was conducted using the TLC–densitometry method. The results demonstrate that the ethyl acetate fraction exhibited the strongest antioxidant activity, with an IC۵۰ value of ۳۷.۱۸ ppm, which is categorized as very strong. This fraction also displayed the highest total phenolic content at ۷.۸۴ g GAE/۱۰۰ g of fraction. However, no direct correlation was observed between phenolic content and antioxidant activity. Additionally, TLC–densitometry analysis revealed that the water fraction exhibited a chromatographic profile similar to ascorbic acid, suggesting the presence of compounds with a comparable structure and antioxidant activity.

کلیدواژه ها:

Pineapple (Ananas comosus L. Merr.) ، Phytochemical profiling ، DPPH Analysis ، TLC Densitometry ، Antioxidant Activity Index