Antidiarrheal Properties and GC-MS Analysis of Methanolic Leaf Extract of Plumeria pudica

The current study assessed and justified the use of a methanolic extract from Plumeria pudica leaves for therapeutic purposes. Mice were used for the in vivo antidiarrheal test, and gas chromatography–mass spectrometry was employed to identify the phytoconstituents. According to the GC-MS data, the main polyphenol found in Plumeria pudica was pyrogallol (۶۴.۰۴ %), along with polysaccharides, triterpenoids, amino acids, steroids, and fatty acid esters. In both doses, ۲۰۰ mg/kg and ۴۰۰ mg/kg, the extract produced a significant reduction in defecation (𝑝 < ۰.۰۱). The leaf extract at ۲۰۰ mg/kg and ۴۰۰ mg/kg decreased the total amount of wet feces produced after the administration of castor oil, with values of ۶.۳۳ ± ۰.۸۳ g and ۵.۶۹ ± ۰.۴۲ g, respectively, compared to the control group, which produced ۵.۰۰ ± ۰.۲۳ g at the dose of ۱۰ mg/kg. Therefore, this work described the chemical makeup of Plumeria pudica and offers mechanistic evidence for its therapeutic application in conditions involving diarrhea and hyperactive gastrointestinal motility.

کلیدواژه ها:

Antidiarrheal activity ، GC-MS ، Plumeria Pudica ، Methanol solvents