Neuroprotective and Antioxidant Properties of Cymbopogon flexuosus: An <em style="mso-bidi-font-style: normal;">In Vitro Evaluation

Neurodegenerative diseases are a growing concern in the biomedical field due to their increasing prevalence and limited treatment options. This study aimed to evaluate the antioxidant properties and neuroprotective potential of Cymbopogon flexuosus leaf extracts using in vitro approaches. Aqueous and ethanolic extracts of Cymbopogon flexuosus leaves were analyzed using GC-MS to identify their phytochemical constituents. Antioxidant activity was assessed through DPPH and ABTS radical scavenging assays. The neuroprotective effect of the extracts was evaluated using the PC-۱۲ neuronal cell line via the MTT assay. Additionally, biochemical parameters including GSH, SOD, AChE activity, and reactive nitrogen intermediates (RNI) were measured. GC-MS analysis revealed the presence of ۱۷ bioactive compounds in the aqueous and ethanolic leaf extracts. Both extracts demonstrated significant antioxidant activity in DPPH and ABTS assays. Treatment with the extracts enhanced intracellular levels of glutathione and SOD while reducing acetylcholinesterase activity and reactive nitrogen intermediates in PC-۱۲ cells. The findings suggest that Cymbopogon flexuosus leaf extracts possess notable antioxidant and neuroprotective properties. These results support the potential application of C. flexuosus in the development of novel therapeutic agents for the management and treatment of neurodegenerative disorders.

کلیدواژه ها:

Anti-oxidant assay GC.MS ، DPPH ، ABTS ، Cymbopogon Flexuous ، Neuroprotective