Determining Some Thermodynamic and Kinetic Parameters of the Effect of Extract Concentration Cupressus sempervirens L. Cone on Free Radical DPPH Inhibition

Antioxidants are compounds that can minimize the effects of free radicals. In recent decades, attention to natural antioxidant compounds has increased due to fewer unwanted side effects compared to artificial antioxidants. This study aimed to investigate the antioxidant and free radical scavenging properties of the hydroalcoholic extract from Cupressus sempervirens L. (C. sempervirens) For this purpose, ۲,۲-diphenyl-۱-picrylhydrazyl (DPPH) method was used to evaluate the antioxidant activity. The reason behind choosing this assay was that using DPPH is the most widely used test. Kinetic and thermodynamic parameters were determined by changing the temperature (at ۳۰۴ - ۳۱۰ K) and recording the spectrum with a UV-Vis spectrophotometer at regular intervals of ۲ min for the first ۱۰ min and, subsequently, every ۵ min until the reaction approaches its steady state. The rate constants (k۲), were obtained as second order. The results also revealed that the increased temperature has a direct effect on the radical scavenging properties of the extract. The value found for activation energy was Ea =۶۹۰.۹۵ kJ.mol-۱. Th hydroalcoholic extract of C. sempervirens is a potential source of natural antioxidants and serves as an effective free radical DPPH scavenger and/or inhibitor. Hence, C. sempervirens might be a good plant-based pharmaceutical product for several diseases caused by free radicals.

کلیدواژه ها:

DPPH ، Cupressus Sempervirens L ، Antioxidant ، Rate constant ، activation energy