Purpose: Salinity stress is one of the most important limiting factors affecting all plant processes, from seed germination to seedling development and plant growth. Seed priming is the most promising seed technique which quickens the germination process and enhances stand establishment. Therefore, seed priming with allantoin and serotonin was performed to investigate their effects on germination and growth of bitter melon seeds under salt. Research method: The experiment was conducted as a factorial experiment in a completely randomized design with three replications. The first factor consisted of three concentrations of salinity (۰, ۵۰ and ۱۰۰ mM NaCl) and the second factor included seed priming (۴۸ h) with modulators: control (distilled water), allantoin (۱۰۰۰ μM), serotonin (۱۰۰ µM) and allantoin + serotonin. After ۴۸ h of applying the priming treatments, the seeds were dried and placed in ۹ cm diameter petri dishes lined with filter paper, moistened with the prepared salt solutions, and allowed to germinate at ۲۵ °C. Germination percentage, seed vigor and selected growth characteristics including length, fresh and dry weight of seedling, shoot and root of bitter melon were evaluated. Findings: The results revealed that seed germination and seedling growth of bitter melon decreased in line with increasing salt concentrations: on the other hand, seed priming with allantoin (۱۰۰۰ μM) and serotonin (۱۰۰ µM) alleviated this negative effect of salt stress. It was concluded that allantoin and serotonin, as seed priming agents, positively influenced bitter melon germination under salt stress. These findings may also be applicable to other plants and probably the observed stress tolerance might occur throughout the plant growth period. Research limitations: No limitations were identified. Originality/Value: Given the medicinal value of bitter melon, solving its germination problem, especially under salt stress conditions, will be of great help to those interested in cultivating this plant.