Assessment of DUSP۱ Expression Levels in Tumor and Surgical Margin Samples of Laryngeal Squamous Cell Carcinoma

Introduction: Laryngeal squamous cell carcinoma remains a major clinical challenge, and conventional margin assessment may overlook molecular alterations associated with residual disease and field cancerization. DUSP۱, a regulator of MAPK signaling, may contribute to tumor behavior. This study aimed to assess DUSP۱ expression levels in tumor and surgical margin samples of LSCC.Material and methods: This descriptive cross-sectional study was conducted in ۲۰۲۴ at Tabriz University of Medical Sciences on ۵۰ patients with laryngeal squamous cell carcinoma, selected through convenience sampling based on the Cochran formula for single-group studies. DUSP۱ expression was measured in paired tumor and surgical margin samples, alongside demographic, clinical, and pathological variables, including age, sex, smoking status, tumor site, grade, TNM stage, lymph node involvement, and margin status.Results: In this study of ۵۰ patients with laryngeal squamous cell carcinoma, mean DUSP۱ expression was significantly higher in tumor tissue than in paired surgical margins (۲.۷۳ ± ۰.۶۸ vs. ۱.۴۱ ± ۰.۳۷; P=۰.۰۰۱). Tumoral DUSP۱ expression was also greater in smokers, poorly differentiated tumors, advanced-stage disease, node-positive cases, and positive surgical margins, indicating a consistent association between elevated DUSP۱ levels and more aggressive clinicopathological characteristics.Conclusion: DUSP۱ was significantly overexpressed in LSCC tumor tissue compared with surgical margins and was associated with adverse clinicopathological features. These findings suggest that DUSP۱ may serve as a promising molecular indicator of tumor aggressiveness and local disease extension.

کلیدواژه ها:

laryngeal squamous cell carcinoma ، DUSP۱ ، Surgical margin ، Gene expression