Examination of ATG۵, ATG۷, and Beclin۱ gene expression in the soleus muscle of animal model Parkinson following eight weeks of endurance training with branched-chain amino acids supplementation

Background: Autophagy is associated with the degradation of intracellular organelles and, as a regulator of cellular homeostasis, can affect muscle atrophy. Therefore, this study aimed to examine ATG۵, ATG۷, and Beclin۱ gene expression in the soleus muscle of an animal model of Parkinson following eight weeks of endurance training with branched-chain amino acids (BCAA) supplementation. Methods: In this study, ۲۵ male Wistar rats (weight = ۱۸۰-۲۰۰ g and age = ۱۲ weeks) were used. First, the rats were divided into five groups (n = ۵): healthy control, Parkinson control, Parkinson + training, Parkinson + BCAA, and Parkinson + training + BCAA. Eight weeks of moderate-intensity endurance training were performed. After anesthetizing the rats, the soleus muscles were isolated; then, RNA extraction, cDNA synthesis, and real-time PCR were used for gene expression analysis. One-way analysis of variance with Tukey's post-hoc test was used to analyze the data (P ≤ ۰.۰۵). Results: ATG۵, ATG۷, and Beclin۱ gene expression in the soleus muscle of rats significantly increased after Parkinson's induction (P-Value = ۰.۰۰۱). After both interventions, there was a significant decrease in the gene expression of ATG۵, ATG۷, and Beclin۱ (P-Value = ۰.۰۰۱), observed only in the endurance training with BCAA supplementation group. Conclusion: Although endurance training and BCAA supplementation alone did not significantly affect ATG۵, ATG۷, and Beclin۱ gene expression, combining both interventions caused a significant decrease, indicating the significant effect of using endurance training with BCAA supplementation for Parkinson's disease in rats.

کلیدواژه ها:

Autophagy ، Autophagy-Related Protein ۵ ، Autophagy-Related Protein ۷ ، Beclin۱ ، Endurance Training