Effects of intraperitoneal administration of kisspeptin-۱۰ on ischemia-reperfusion injury in a rat testicular torsion and detorsion model

Testicular ischemia-reperfusion injury during testicular torsion is strongly influenced by oxidative stress caused by excessive accumulation of uncaptured reactive oxygen species. Kisspeptin-۱۰ (Kiss-۱۰), a biologically active fragment of kisspeptin peptide family, has demonstrated significant anti-oxidant and anti-apoptotic properties. Recent studies indicate that Kiss-۱۰ can mitigate oxidative stress by reducing reactive oxygen species levels and enhancing the activity of endogenous anti-oxidant enzymes, such as superoxide dismutase and catalase. This study examined the effects of Kiss-۱۰on ischemia-reperfusion injury in testicular torsion/detorsion (T/D) of male rats. Twenty male rats were divided into four groups, including the control group and three treatment groups (۷۲۰° T/D, ۷۲۰° T/D + ۰.۵۰ µg kg-۱ Kiss-۱۰, and ۷۲۰° T/D + ۱.۰۰ µg kg-۱ Kiss-۱۰). After inducing ۷۲۰° clockwise testicular torsion for ۲ hr, sperm parameters, such as number, motility, viability, DNA damage, and hypo-osmotic test were evaluated. The results showed that ۷۲۰° T/D can increase sperm DNA damage. Also, it also had negative effects on overall motility and other properties, such as viability and plasma membrane functionality. The results also showed that administration of Kiss-۱۰to T/D rats can reduce DNA damage. It could also increase overall motility, viability, and plasma membrane functionality compared to the T/D group. Based on our results, Kiss-۱۰ provides significant protection against acute T/D injury to the testis when administered after spermatic cord torsion in rat.

کلیدواژه ها:

ISCHEMIA-REPERFUSION ، Kisspeptin-۱۰ ، Rat ، Sperm ، Testis