Molecular investigation of biofilm-forming genes in staphylococci isolated from dogs in Ilam, Iran

Biofilm formation is a key virulence factor in Staphylococcus aureus, contributing to bacterial persistence, antimicrobial resistance, and chronic infections. This study aimed to investigate the presence of biofilm-associated genes (fib, fnbA, fnbB, clfA, and clfB) in S. aureus isolates from dogs in Ilam, Iran. From December ۲۰۲۲ to September ۲۰۲۳, ۲۵۰ swab samples were collected from nasal, oral, and rectal sites of dogs, yielding ۸۱ S. aureus isolates confirmed by PCR amplification of the nuc gene. The prevalence of biofilm-associated genes varied, with clfA, clfB, and fnbA detected in ۹۸.۸۰% of isolates, fib in ۶۳.۰۰%, and fnbB in ۱۶.۰۰%. Notably, fnbA, clfA, and clfB were present in all rectal isolates, while fnbB was absent in this group. The findings highlighted the widespread presence of biofilm-related genes in S. aureus from dogs, suggesting their potential role in colonization and zoonotic transmission. The high prevalence of adhesion-associated genes underscored the need for monitoring biofilm-forming S. aureus in companion animals to mitigate antimicrobial resistance and public health risks.

کلیدواژه ها:

biofilm ، Dogs ، Staphylococcus aureus ، Virulence Factors ، zoonoses