<span lang="EN-SG">Polymorphisms of the Self-Incompatibility Locus of Sweet Cherry (<em style="mso-bidi-font-style: normal;">Prunus avium L.) Species from the VNIISPK Bio-Resource Collection

Self-incompatibility is a common evolutionary mechanism of flowering plants. Sweet cherry (Prunus avium L.) has a gametophytic type of self-incompatibility. Most of sweet cherry cultivars are self-incompatible, although self-compatible genotypes also occur. This study presents data of S genotype investigation of ۳۹ sweet cherry cultivars from VNIISPK bio-resource collection. To identify S genotypes, PCR with consensus (PaConsI and PaConsII) and allele-specific (S۱, S۲, S۳, S۴, S۵, S۶, S۷, S۸, S۹, S۱۰, S۱۱, S۱۲, S۱۳, S۱۴ and S۱۶) primers was done. The S genotype was fully identified for ۳۳ cultivars. Then, ۷ S alleles (S۱, S۳, S۴, S۵, S۶, S۹, and S۱۳) were detected in the genomes of the investigated sweet cherries. According to the results of allele-specific amplification, ‘Venera’ (S۱x/S۴), ‘Zolotuhinskaya’ (S۴x/S۶) and ‘Viskochka’ (S۳/S۴x) were suspected to have unique or mutant alleles of the gene. The cultivars with fully determined S-genotypes were distributed into nine incompatibility groups (III, VI, VII, IX, XV, XVII, XIX, XXI, and XLV).

کلیدواژه ها:

consensus primers ، allele-specific primers