In vitro efficacy of Ziziphus vulgaris L. and Camellia sinensis L. extracts against Giardia duodenalis cysts

There is an increasing interest in exploring plant extracts as potential new treatment options for these ailments. Ziziphus vulgaris L. and Camellia sinensis (L.) Kuntze are medicinal plants widely used in different traditional medical practices, especially in Iranian traditional medicine, for addressing a range of health issues. Although their biological properties are well-established, their effectiveness against Giardia duodenalis cysts is still uncertain. This research aimed to assess the impact of Z. vulgaris and C. sinensis extracts on G. duodenalis cysts in vitro and to compare their effectiveness with metronidazole. Cysts were extracted from stool samples and concentrated using a ۰.۸۵ M sucrose solution. Extracts of Z. vulgaris and C. sinensis were prepared at concentrations of ۲۵.۰۰, ۵۰.۰۰, and ۱۰۰ mg mL-۱. The impacts of these extracts at different concentrations were evaluated at ۱۰, ۱۵, ۳۰, ۶۰, and ۱۸۰ min, and the results were compared to control groups. The collected data were documented and statistically analyzed. The findings revealed that Z. vulgaris extract at a concentration of ۱۰۰ mg mL-۱ and C. sinensis extract at a concentration of ۵۰.۰۰ mg mL-۱ showed similar effectiveness to metronidazole in eliminating Giardia cysts. In addition, the cytotoxic effects of Z. vulgaris and C. sinensis extracts, in comparison with metronidazole, indicated a rise in fatality rates with prolonged exposure times and higher extract concentrations. Therefore, it could be concluded that extracts of Z. vulgaris and C. sinensis were as effective as metronidazole for killing Giardia cysts in vitro.

کلیدواژه ها:

Camellia sinensis ، Cyst ، Giardia duodenalis ، Ziziphus vulgaris