Screening of Some Rare and Endemic Plants Growing in Iran for Their Cytotoxic Effects on Cancer Cell Lines

Khorasan Razavi Province is one of the most significant regions of plant diversity of the flora of Iran. Numerous rare indigenous plant species are grown in this province, which would be suitable potential candidates for assessing cytotoxic activities and apoptosis induction. In this study, we intended to explore the cytotoxic and apoptotic properties of the methanol extract of the root and aerial parts of ۱۰ rare and endemic plants of Khorasan Razavi Province, namely Dorema kopetdaghense, Chaerophyllum khorassanicum, Seseli staurophyllum, Seseli transcaucasicum, Haplophyllum obtusifolium, Crinitaria grimmii, Handelia trichophylla, Rheum turkestanicum, Phlomoides boissieriana., and P. molucelloides, on PC-۳, MCF-۷, MDA, and B۱۶F۱۰ cancer cells using resazurin and flow cytometry assays. ۱۰۰ μg/ml of F.kopetdagi root extract on PC۳ (p <۰.۰۰۱), MCF-۷ (p <۰.۰۰۱), MDA (p <۰.۰۰۱), B۱۶F۱۰ (p <۰.۰۰۱) cells significantly decreased cell viability. ۱۰۰ μg/ml of H. obtusifolium root extract on PC۳ (p <۰.۰۰۱), MCF-۷ (p <۰.۰۰۱), MDA (p <۰.۰۵), B۱۶F۱۰ (p <۰.۰۰۱) cells, and ۵۰ μg/ml of H. obtusifolium root extract on MCF-۷ (p <۰.۰۱), B۱۶F۱۰ (p <۰.۰۰۱) exhibited cytotoxic effects. The histogram obtained from the PI staining and flow cytometry indicated induction of apoptosis by ۲۵ and ۵۰ μg/ml F.kopetdagi and H. obtusifolium root extracts in the PC۳ and MCF-۷ cell lines. The root extracts of F.kopetdagi and H. obtusifolium had significant cytotoxic effects and could induce apoptosis. It suggests that plants with low IC۵۰ values are considered potent cytotoxic species for further evaluation as candidate anti-cancer agents.

کلیدواژه ها:

Ferula kopetdagi ، Haplophyllum obtosifolium ، Cytotoxic ، Apoptosis