Chemical Mechanisms of Sodium Tanshinone IIA Sulfonate in Modulating SIRT۱-Mediated Inflammation and Oxidative Stress

Sodium Tanshinone IIA Sulfonate (STS), a sulfonated derivative of tanshinone IIA with the molecular formula C۱۹H۱۷NaO۶S and a molecular weight of ۳۹۶.۶۹ g/mol, exhibits significant anti-inflammatory and antioxidant properties due to its sulfonate group, which enhances water solubility and bioavailability. This study investigated the chemical mechanisms of STS in Coronary Heart Disease (CHD) via SIRT۱-mediated pathways. First of all, the CHD rat model was established by left coronary artery ligation combined with ischemia/reperfusion injury. The rats were randomly divided into control, model, low (۱۰ mg/kg), medium (۲۰ mg/kg), and high (۴۰ mg/kg) dose STS groups, with chemical purity >۹۸%. Liver function test was used to determine the optimal dose of STS intervention (۲۰ mg kg), Western blot (WB) was used to detect the expression of SIRT۱ protein, tissue staining (HE, oil red O) was used to observe the atherosclerotic lesions, and cardiac function was evaluated by a cardiac function instrument. Results showed STS dose-dependently upregulates SIRT۱ expression (P<۰.۰۵), reduces atherosclerotic plaque formation (oil red O staining), and improves hemodynamics by modulating NF-κB and Nrf۲ pathways. STS at ۲۰ mg/kg demonstrated optimal efficacy with minimal hepatotoxicity. These findings highlight STS's role in regulating inflammation and oxidative stress via SIRT۱, providing a basis for its use in cardiovascular therapy.

کلیدواژه ها:

Coronary heart disease ، Sulfonation of tanshinone IIA ، chemical analysis ، SIRT۱ ، Sodium tanshinone IIA sulfonate