Loading of Cisplatin Drug into Magnesium Ion-Doped Hydroxyapatite Nanoparticles in the Presence of a Magnetic Field: Fabrication and Chemical Analysis

This study aimed to develop magnesium-doped hydroxyapatite (HA) nanoparticles as a novel drug delivery system for the anticancer agent cisplatin, with investigation of magnetic field effects on drug loading and release characteristics. Methods: Magnesium-doped HA nanoparticles with ۵%, ۱۰%, and ۱۵% magnesium content were synthesized via sol-gel and co-precipitation methods. The nanoparticles were characterized using Fourier Transform InfraRed (FT-IR) spectroscopy, Scanning Electron Microscopy (SEM), Energy-Dispersive X-ray spectroscopy (EDX), Dynamic Light Scattering (DLS), and zeta potential analysis. Cisplatin loading was performed using the water-in-oil emulsion method under a static magnetic field (۰.۵ Tesla). Drug release profiles were evaluated over ۷۲ hours in simulated body fluid using ultraviolet-visible spectroscopy (UV-Visible). Successful incorporation of magnesium ions into the HA lattice was confirmed by FT-IR peak shifts and EDX analysis. The synthesized nanoparticles exhibited uniform morphology with diameters ranging from ۵۰–۱۵۰ nm. This study aimed to develop magnesium-doped hydroxyapatite (HA) nanoparticles as a novel drug delivery system for the anticancer agent cisplatin, with investigation of magnetic field effects on drug loading and release characteristics. Methods: Magnesium-doped HA nanoparticles with ۵%, ۱۰%, and ۱۵% magnesium content were synthesized via sol-gel and co-precipitation methods. The nanoparticles were characterized using Fourier Transform InfraRed (FT-IR) spectroscopy, Scanning Electron Microscopy (SEM), Energy-Dispersive X-ray spectroscopy (EDX), Dynamic Light Scattering (DLS), and zeta potential analysis. Cisplatin loading was performed using the water-in-oil emulsion method under a static magnetic field (۰.۵ Tesla). Drug release profiles were evaluated over ۷۲ hours in simulated body fluid using ultraviolet-visible spectroscopy (UV-Visible). Successful incorporation of magnesium ions into the HA lattice was confirmed by FT-IR peak shifts and EDX analysis. The synthesized nanoparticles exhibited uniform morphology with diameters ranging from ۵۰–۱۵۰ nm. DLS analysis revealed average particle sizes of ۷۵ nm for undoped HA and ۱۴۴ nm for magnesium-doped HA, with zeta potential values of −۱۹ mV and −۱۵.۵ mV, respectively. Cisplatin loading efficiency was enhanced in the presence of the magnetic field. Drug release studies demonstrated that magnesium-doped HA nanoparticles achieved significantly higher and more sustained cisplatin release compared to undoped HA, with the magnetic field further improving release kinetics from magnesium-doped HA formulations over ۷۲ hours. Magnesium-doped HA nanoparticles, particularly when combined with magnetic field application during drug loading, represent a promising platform for controlled cisplatin delivery with potential for targeted cancer therapy. This approach addresses limitations of conventional cisplatin chemotherapy by offering improved drug loading, sustained release, and reduced systemic toxicity.

کلیدواژه ها:

Magnesium-doped hydroxyapatite ، cisplatin ، Magnetic field ، nanoparticles ، Controlled release.