UV-Vis Spectrophotometric Analysis of Butyl Methoxy Dibenzoyl Methane in Sunscreen Products: A Method Validated to Cosmetic Industrial Interests

Sunscreen is applied for protection against adverse effects from sunlight onto skin. Sun protection efficiency can be examined in accordance with the industrial standards for cosmetics. In vivo testing, the gold standard of testing, leads to many drawbacks that may impede the assured quality. Accordingly, an accurate and reliable in vitro testing is regarded an efficient tool feasibly for the industrial practice, specifically chemical analysis of the sunscreen. A validated UV-Vis spectrophotometric method for sunscreen formulation was therefore developed to meet with the industrial perspectives for quality practices. Butyl methoxy dibenzoyl methane (BMDM), the most commonly used sunscreen, was subjectively validated in this study. Ethanol was indicated as the suitable solvent with a linearity of ۰.۹۹۹ and analytical range of ۲.۵ - ۷.۵ µg/mL. The method was indicated to be accurate and precise to determine and analyze low, intermediate and high BMDM concentrations giving ۹۷.۵۹ - ۱۰۱.۸۸% recoveries and relative standard deviation of less than ۲% (۰.۸۵ - ۱.۵۳%) that are in line with the acceptable limits of the guidelines. This method was consequently specified for BMDM (۳%, w/w) in an emulsion (o/w) as exhibited with the recovery and the relative standard deviation determined in the freshly prepared and after the stability test (۱۲۰.۲۰ ± ۲.۱۶% and ۱.۸۰%, as well as ۱۰۳.۱۳ ± ۲.۰۷% and ۲.۰۰%, respectively) products. The validated and cost-efficient spectrophotometric method presented is significantly feasible for the industrial practice, and the quality of the sunscreen is warranted.

کلیدواژه ها:

Validated UV/VIS-spectrophotometry ، Sunscreen products ، Sunscreen testing ، Cosmetic analysis ، Quality Control ، Quality Assurance