Nanoencapsulation of A. paniculata and A. scholaris Extracts in Carboxymethyl Chitosan to Enhance Antibacterial Activity against E. coli

Antibiotic resistance against E. coli is a serious challenge in the treatment of infectious diseases. A. paniculata and A. scholaris are medicinal plants known to have antibacterial activity, but their utilization is hampered by low bioavailability. This study aims to develop a nanoencapsulation system of A. paniculata and A. scholaris extracts using a carboxymethyl chitosan (CMC) matrix to enhance antibacterial activity against E. coli. Physicochemical characterization includes particle size, zeta potential, encapsulation efficiency, and extract release profile. Antibacterial activity was evaluated through the inhibition zone diameter test. Results showed that the comparison of herbal combination extract and CMC has an effect on pH stability, loading, release, and antibacterial activity against E. coli. Based on the FTIR results, it shows the success of encapsulation through the interaction in the form of hydrogen bonds and the absence of the formation of other new bonds. EH-NPs have a particle size of ۴۹۲.۱۹ nm and a PDI of ۰.۳۴۶۴ which has met the criteria for good nanoparticles. The SEM-EDX results show irregular spherical particle morphology, uneven, porous surfaces, even distribution of elements (C ۶۰%, O ۴۹%, and N ۰.۵%), and no contamination.

کلیدواژه ها:

Infectious Disease ، Carboxymethyl chitosan ، Antibiotic Resistance ، A. paniculata ، A. scholaris