Development of a rapid and sensitive electrochemical sensing assay by applying metal-organic framework/multi-walled carbon nanotubes (MOF/MWCNTs) for determination of ascorbic acid

Objectives: L-ascorbic acid (AA, vitamin C), has important bodily functions, including immune defense, collagen formation, and metabolism. Symptoms of many diseases like cancer or cardiovascular disorders can be observed owing to maladjustment or deficiency. Hence, developing sensitive and rapid methods to determine AA in biological samples is essential. In this study, a novel electrochemical sensing assay based on Zn metal-organic framework embedded in multi-walled carbon nanotubes (Zn-MOF/MWCNTs) has been effectively developed to modify glassy carbon electrode (GCE).Materials and Methods: Zn-MOF was synthesized using the solvothermal technique and mixed with multi-walled carbon nanotubes to obtain a modifier suspension. Various characterization methods including XRD (X-ray Diffraction), EDX (Energy-dispersive X-ray spectroscopy), HR-TEM (High-resolution transmission electron microscopy), FT-IR (Fourier-transform infrared spectroscopy), and FESEM (Field emission scanning electron microscopy) were applied to confirm the proper synthesis of Zn-MOF. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) approaches were employed to study the electrochemical behavior of the modified GCE and electrochemical determination of AA with high sensitivity.Results: Under the optimized conditions including the drop volume, supporting electrolyte type and concentration, buffer type and concentration, and pH, the linear range and LOD (detection limit) were acquired ۲–۲۲ μM equal to ۱.۲۱۱ μM respectively. Moreover, other prominent analytical features including repeatability, stability, and high reproducibility were investigated for the proposed sensing platform. Additionally, the prepared sensor was effectively utilized for determination of AA in human plasma samples, attaining a recovery of ۹۳.۱%.Conclusion: These findings clearly confirm that the developed Zn-based MOF/MWCNT sensing assay is a promising platform for accurate and effective AA determination in real biological samples, and confirms its potential for feasible biomedical applications.

کلیدواژه ها:

Ascorbic acid ، Electrochemical sensing assay ، Zn-based Metal-organic framework ، Multi-walled carbon nanotubes ، Modified electrodes