Synergistic nanomedicine: Catechin@Cerium-hydroxyapatite nanoparticles for oxidative stress mitigation and concurrent osteosarcoma ablation and Osteogenesis

Objective(s): A significant problem in osteosarcoma treatment and bone regeneration is the creation of biomaterials that efficiently target cancer cells while concurrently facilitating bone tissue regeneration. We introduce an innovative dual-action nanomedicine, catechin-functionalized nano cerium-hydroxyapatite (CH@Ce-HA), aimed at addressing these constraints by integrating cytotoxic and regenerative capacity.Materials and Methods: The CH@Ce-HA composite was created by integrating the natural anticancer and antioxidant compound, catechin, onto a nanoscale cerium-doped hydroxyapatite matrix. The physicochemical analysis via FT-IR revealed the incorporation of catechin, while XRD validated the retention of the HA crystalline structure. TEM examination demonstrated a homogeneous distribution of nanoparticles ranging from ۲۰ to ۵۰ nm in size. The combined therapeutic efficacy was further examined in vitro utilizing osteosarcoma and osteoblast cell lines, evaluating cell proliferation, oxidative stress reduction, apoptosis induction, and osteogenic differentiation.Results: In vitro investigations utilizing osteosarcoma and osteoblast cell lines demonstrated the nanocomposite's exceptional therapeutic flexibility. The CH@Ce-HA demonstrated improved antioxidant activity and a strong nanomedicine effect against MG۶۳ osteosarcoma cells, markedly suppressing proliferation, diminishing harmful oxidative stress, and effectively inducing apoptosis. The nanocomposite significantly improved osteoblast proliferation, differentiation, and mineralization.Conclusion: This study presents a potential, multifunctional ceramic based therapeutic agent that functions at the nanoscale to offer a comprehensive treatment for bone oncology. The CH@Ce-HA nanocomposite integrates targeted cytotoxic effects with robust osteogenic and antioxidant properties, marking a notable progress in translational nanomedicine treating challenging bone abnormalities and cancers.

کلیدواژه ها:

Osteosarcoma ، Bone Repair ، Catechin ، Osteoblast proliferation ، Anti-oxidant activity