Histopathological Investigation of the Anti-inflammatory Effects of Metformin in an Experimental Model of Chronic Non-bacterial Prostatitis in Rats

Background: Chronic prostatitis is one of the most common diseases of the prostate gland, significantly impacting men’s quality of life. Today, metformin is recognized as a medicine with multiple therapeutic potentials, including enhanced anti-inflammatory and antioxidant activity, antitumor effects, benign prostatic hyperplasia, etc. Objectives: The present study aimed to investigate the histopathological effects of metformin on chronic non-bacterial prostatitis (CNP) induced by carrageenan in rats.Methods: Twenty-four male rats were divided into four groups (n=۶): Group I (Sham: pseudo CNP-untreated), group II (control: CNP-untreated), group III (cernilton: CNP-standard treatment), and group IV (MET: CNP-under treatment). To induce CNP, ۰.۱ mm carrageenan (۱%) was injected into the prostate of groups II, III, and IV. In contrast, an equal amount of normal saline was injected into group I. Subsequently, group IV was treated with MET (۱۰۰ mg/kg), group III with cernilton (۱۰۰ mg/kg), and groups I and II with ۰.۵ mL normal saline, orally daily for up to ۳ weeks (۲۱ days). After ۲۱ days, the rats were euthanized, and prostate tissue sections were prepared for histological staining with hematoxylin-eosin.Results: Group IV had a significant difference in inflammation compared to group II (P<۰.۰۵). Additionally, from a histopathological perspective, group IV exhibited a significant reduction in inflammation but in hyperplasia of acinar epithelial cells and hyperemia. Although there were no statistically significant differences among all groups, the reduction of hyperplasia and the increase in hyperemia were notable in group IV, with the tissue appearance closely resembling that of normal prostate tissue.Conclusion: In the present study, it was demonstrated for the first time that metformin reduces tissue inflammation and its associated complications in the prostate.

کلیدواژه ها:

Chronic Prostatitis ، Histopathology ، Metformin