Dorema aucheri extract for reducing oxidative stress and kidney injuries in diabetic rats

Objective: Diabetic hyperglycemia causes oxidative stress, contributing to chronic kidney disease (CKD). This study evaluated the effects of ethanolic Dorema aucheri extract from aerial parts on oxidative stress, inflammation, and kidney injury in diabetic rats.Materials and Methods: Thirty male rats were randomly assigned to five groups (n=۶): non-diabetic control, diabetic control, diabetic + metformin (۵۰۰ mg/kg/day), and diabetic + D. aucheri extract (۲۵۰ or ۵۰۰ mg/kg/day). Treatments were given orally for ۲۸ days. Serum and kidney samples were analyzed for fasting blood glucose (FBS), renal function [blood urea nitrogen (BUN), creatinine (Cr)], inflammatory cytokines [interleukin-۶ (IL-۶), interleukin-۱β (IL-۱β)], oxidative stress [malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx)], mRNA expression [kidney injury molecule-۱ (KIM-۱), neutrophil gelatinase-associated lipocalin (NGAL), B-cell lymphoma ۲ (BCL-۲), Caspase-۹ (CASP-۹)], stereology, and histopathology.Results: Treatment with D. aucheri hydroalcoholic extract significantly improved metabolic, inflammatory, oxidative, and renal outcomes in diabetic rats. Specifically, both ۲۵۰ mg/kg and ۵۰۰ mg/kg doses reduced blood glucose (p<۰.۰۵), BUN (p<۰.۰۵ and p<۰.۰۱) and Cr (p<۰.۰۱ for ۵۰۰ mg/kg), IL-۶ (p<۰.۰۵), and IL-۱β (p<۰.۰۵ and p<۰.۰۰۱), respectively. MDA decreased (p<۰.۰۰۱ and p<۰.۰۵), SOD, CAT, and GPx increased (p<۰.۰۱). Renal KIM-۱ and NGAL decreased (p<۰.۰۱), CASP-۹ decreased (p<۰.۰۱ and p<۰.۰۵), and BCL-۲ increased (p<۰.۰۵), respectively. Histopathology confirmed reduced kidney damage correlating with biochemical and gene expression improvements.Conclusion: Dorema aucheri extract has the potential to modulate antioxidant and apoptotic pathways and reduce kidney injury in diabetic rats. Further studies are needed to confirm its therapeutic efficacy.

کلیدواژه ها:

Dorema aucheri ، Oxidative stress ، Antioxidant ، Inflammation ، Diabetes