The hepatoprotective impacts of Citrullus colocynthis seeds extract on type ۱ diabetes-induced liver inflammation and oxidative stress in rats: The role of nrf۲/keap۱ pathway

Objective: Type ۱ Diabetes (T۱D) induces several consequences including hepatic damage resulting from oxidative stress and inflammation. The Nrf۲/Keap۱ pathway is crucial in oxidative stress signaling, and its modifications significantly influence health and illness. In this study, rats with T۱D were used to test Citrullus colocynthis (CC) ability to reduce oxidative stress and inflammation in the diabetic liver.Materials and Methods: Twenty-one male Wistar rats were assessed. Three groups—control (healthy rats), diabetes (received STZ), and diabetes with drug—were allocated at random to the rats. The CC fruits extract was administered to diabetic rats via gavage for ۴۰ days. Oxidative stress indicators, including malondialdehyde (MDA), total antioxidant capacity (TAC), and superoxide dismutase (SOD), were determined. The ELISA method quantified liver cytokines, whereas western blotting evaluated the Nrf۲/Keap۱ pathway. Results: The administration of CC in the Diabetes+Drug group dramatically reduced MDA levels while enhancing SOD activity and TAC levels in the liver. Following CC administration, Tumor necrosis factor alpha (TNF-α) levels decreased while rats with diabetes had higher amounts of interleukine-۱۰ (IL-۱۰) in their liver tissue. The CC administration could regulate the Nrf۲/Keap۱ pathway.Conclusion: This study concluded that a daily administration of ۲۰۰ mg/kg CC for ۴۰ days can enhance liver function in diabetic subjects by controlling the Nrf۲/Keap۱ pathway and reducing oxidative stress and inflammation.

کلیدواژه ها:

Citrullus colocynthis ، Type ۱ diabetes ، Nrf۲/Keap۱ pathway ، Oxidative stress ، Inflammation