Determination of chlorpheniramine maleate via the new approach of ion pair/turbidimetric reaction with continuous flow injection analysis, and validated by UV-Vis spectrophotometry

Chlorpheniramine maleate was analyzed using an AYAH ۶SX۱-۱D instrument to develop a turbidimetric continuous-flow injection method (T-CFIA) based on its reaction with sodium tungstate dihydrate as a precipitating reagent in an acidic medium. The physical and chemical parameters were optimized, establishing a linear range of ۰.۶-۳۰ mmol L-۱, a limit of detection (LOD) of ۲۵۰ nmol L-۱, and a repeatability relative standard deviation percentage of <۰.۵%. A comparative analysis using UV-Vis spectrophotometry at λmax = ۲۶۵ nm revealed no significant difference at the ۹۵% confidence level, demonstrating the reliability of the proposed method for detecting chlorpheniramine maleate. The paired t-test showed that the developed method is comparable to UV-Vis spectrophotometry, confirming its reliability for determining chlorpheniramine maleate. The developed method was applied to determine chlorpheniramine maleate in three pharmaceutical samples. This method offers a sensitive and accurate approach to determining chlorpheniramine maleate.

کلیدواژه ها:

Chlorpheniramine maleate ، Turbidimetric method ، flow injection ، AYAH ۶SX۱-۱D Analyzer ، UV-Vis spectrophotometry