Investigating the effects of taurine on Aβ-degradation proteins and neurogenesis-related genes in an LPS-induced neuroinflammatory SH-SY۵Y cell model

Objective(s): Taurine, a brain-abundant sulfonic acid, shows potential neuroprotective effects and may counter neurodegenerative processes. This study investigated the effects of taurine on neuroinflammation, focusing on Aβ-degrading proteins and gene expression related to neurogenesis in an LPS-stimulated SH-SY۵Y cell model.Materials and Methods: The effects of taurine (۰.۵ and ۱ mg/ml) and LPS (۰.۵ and ۱۰ μg/ml) on SH-SY۵Y cell viability were assessed using the MTT assay. IL-۱β and IL-۶ expression was measured by real-time PCR, while matrix metalloproteinases (MMPs) and angiotensin-converting enzyme (ACE) levels were quantified via ELISA. Neurogenesis-related gene expression was evaluated using the Neurogenesis Plus RT² Profiler PCR array.Results: The MTT assay results demonstrated that taurine treatment at concentrations of ۰.۵ and ۱ mg/ml significantly attenuated the cytotoxicity induced by LPS stimulation in SH-SY۵Y cells. Also, treatment with taurine significantly reduced the expression levels of the inflammatory genes IL-۱β and IL-۶ in LPS-stimulated SH-SY۵Y cells (P<۰.۰۵). ELISA results further revealed that taurine treatment significantly increased the secretion levels of ACE and MMP-۹ enzymes in LPS-stimulated SH-SY۵Y cells (P<۰.۰۵). LPS exposure in SH-SY۵Y cells significantly up-regulated genes related to apoptosis, cell migration, and synaptic function (P<۰.۰۰۵). Conversely, taurine treatment significantly increased the expression of genes involved in cell adhesion, synaptic function, growth factors, cytokines, differentiation, cell cycle, signaling, transcription, and cofactor activity (P<۰.۰۰۵).Conclusion: These results suggest that taurine may act as a potential neuroprotective agent by increasing the secretion of MMP-۹ and ACE, regulating neurogenesis-related genes, and reducing LPS-induced neuroinflammation.

کلیدواژه ها:

Lipopolysaccharides ، Matrix metalloproteinase ۹ ، Neurogenesis Neuroinflammatory - diseases ، Neuroprotective agents Taurine