Evaluation of Anti-Asthmatic Potential of Emblica Officinalis Seed Extract in RAW ۲۶۴.۷ Cell Lines

This study aims to explore the anti-asthmatic potential of Emblica officinalis seed extract by assessing its effect on inflammatory markers in RAW ۲۶۴.۷ cell line. The preliminary evaluation of Emblica officinalis seed extract performed and characterized by UV, HPLC, and GCMS. Molecular docking was carried out using Schrodinger. DPPH, ABTS, and nitric oxide production were determined to study antioxidant effects, and cytotoxicity was assessed by MTT assay. The expression of IL-۴, IL-۱۳, TNF-alpha in LPS induced RAW ۲۶۴.۷ cell lines was analyzed using FACS method. The hydroalcoholic extract produced an extractive and ash values of ۲.۸۵% w/w and ۳.۴۷ ± ۰.۱۵% w/w, respectively. The determination of total phenolic and flavonoid content was favorable. The characterization of the extract was performed and analyzed through HPLC chromatograms. The GCMS chromatogram showed peaks at various retention times indicating the presence of phytoconstituents. In silico studies showed good interactions between selected ligands and the target proteins. The antioxidant studies proved that the extract possesses free radical scavenging activity, reduces nitric oxide production, and MDA levels. The extract proved to be non-toxic in the MTT assay. In LPS induced RAW ۲۶۴.۷ cells the expression of the target proteins that actively involved in the pathogenesis of asthma was decreased.

کلیدواژه ها:

Antiasthmatic activity ، Emblica officinalis ، Seed extract ، RAW ۲۶۴.۷ cell line ، LPS induced