Staphylococcus Aureus Isolated from Surgical Site Infections: Evaluation of Biofilm and Biofilm-Related Genes

Introduction: Staphylococcus aureus is the most common bacterium causing surgical site infections (SSIs). The biofilm formation of S. aureus leads to increased survival and bacterial persistence. This study aimed to evaluate the frequency of biofilm formation and the presence of icaD, icaA, fnbA, clfA, and cna genes in S. aureus from SSIs isolated from patients in two hospitals in southwest Iran. Methods: This cross-sectional study included ۷۷ S. aureus isolates from SSIs. The ability of S. aureus for biofilm formation was assessed by the Congo Red Agar method. The prevalence of icaD, icaA, clfA, fnbA, cna, and mecA genes was determined by PCR. Data were analyzed using SPSS software (version ۲۰) through chi-square test and Fisher's exact tests. Results: Biofilm formation was observed in ۵۵ isolates (۷۱.۴%), including ۳۰ (۵۴.۵%) strong and ۲۵ (۴۵.۵%) weak biofilm producers’ isolates. The ability to form biofilm was higher among MRSA isolates (۸۳.۷%) compared to MSSA isolates (۵۰%). The frequency of the icaD, fnbA, clfA, cna, and icaA genes was ۶۲.۳%, ۴۲.۹%, ۲۸.۶%, ۲۰.۸%, and ۱۹.۵%, respectively. There was a significant association between the presence of fnbA (P=۰.۰۰۰), clfA (P=۰.۰۰۰), icaD (P=۰.۰۲) genes and biofilm formation; however, it was not observed for icaA (P=۰.۰۵۴), and cna (P=۰.۱۳۲) genes. Conclusions: Our findings reinforce the role of Biofilm-related genes (fnbA, clfA, icaD) and biofilm formation. Given the role of genes in biofilm formation, we can pursue the development of scientific solutions to control biofilms, which ultimately lead to improved public health. Key words: Polymerase Chain Reaction, Staphylococcus aureus, Surgical Wound Infection

کلیدواژه ها:

Polymerase Chain Reaction ، Staphylococcus aureus ، Surgical Wound Infection