The effect of eight weeks of high-intensity interval training on gene expression and serum levels of HGPRT enzyme, uric acid and liver biomarkers in Khorasan wrestlers

Wrestling is a high-intensity sport in which the anaerobic system plays an important role. In this study, we studied the effect of eight weeks of high-intensity interval training on gene expression and serum levels of HGPRT enzyme, uric acid and liver biomarkers in Khorasan wrestlers. Research Methods: In this study, ۱۷ wrestlers with a mean age of (۲۲±۲.۳۲) years. Weight (۷۵.۸ ± ۸.۸ kg), height (۱۷۳.۶ ± ۴.۸ cm) participated and were randomly divided into two groups of high-intensity training (Lemaire protocol) and traditional wrestling training. The training group used the high-intensity protocol (Lemaire) to measure selected biomarkers. Blood samples were taken before and immediately after the high-intensity training. Statistical analysis was performed using SPSS version ۲۲ software. The Shapiro-Wilk statistical method was used for data normality and the Levon test for homogeneity of variances. Dependent t-test and independent t-test were used to examine pre- and post-group and intergroup changes. The significance level in all statistical tests was considered ۰.۰۱. Results: The results of the present study showed that the high-intensity training protocol (Lemaire protocol) after eight weeks of training caused significant intra-group changes in the variables: serum levels of HGPRT, ALT and AST enzymes and no significant changes in HGPRT gene expression and serum uric acid. Khorasani elite wrestlers. .The results also showed that within-group changes in the traditional wrestling training protocol in HGPRT gene expression, serum levels of HGPRT enzyme, ALT and AST enzymes were significant, but it was found that serum levels of uric acid were not significant. Also, the research results showed that between-group changes only showed ALT and AST liver enzymes, which by examining the averages of the groups before and after the intervention showed a significant increase in both enzymes in the traditional training group and a significant decrease in both enzymes in the Lemaire training group.