In vitro exploration of the therapeutic potential of mesenchymal stem cell conditioned medium in endometriosis treatment by targeting apoptosis and cellular migration

Background: Endometriosis, a common gynecological disorder involving ectopic endometrial tissue, leads to infertility and chronic pain. Dysregulated apoptosis and abnormal cell migration are key pathological features. Given current treatment limitations, novel strategies like mesenchymal stem cells (MSC) derived conditioned media (CM) are explored due to their rich secretome. Objective: To investigate the effects of CM from healthy menstrual blood-derived MSCs (MenSCs-CM) and human adipose tissue-derived MSCs (ADSCs-CM) on apoptosis and migration in endometriotic MenSCs. Materials and Methods: This in vitro study involved the isolation of endometriotic MenSCs from infertile women (۲۵-۳۵ yr) via menstrual blood. Mononuclear cells were cultured to passage ۳, and characterized using flow cytometry (CD۲۹, CD۴۴, CD۷۳, CD۱۰۵ positive; CD۳۴, CD۳۸, CD۴۵ negative). CM was prepared separately from healthy donor MenSCs and ADSCs. Endometriotic MenSCs were treated with healthy MenSCs-CM and ADSCs-CM independently. Cell migration (scratch assay), apoptosis (Annexin V), and Bax mRNA levels and Bax/Bcl‑۲ ratio (real-time polymerase chain reaction) were evaluated. Results: Both ADSCs-CM and MenSCs-CM significantly increased during early and late apoptosis in endometriotic MenSCs (p < ۰.۰۰۱). Scratch assays showed significantly decreased MenSCs migration at ۲۴, ۴۸, and ۷۲ hr (p < ۰.۰۰۱, p = ۰.۰۰۳, p < ۰.۰۰۱, respectively). Gene expression revealed significant increases in Bax mRNA (p = ۰.۰۱۳) and the Bax/Bcl‑۲ ratio (p = ۰.۰۴۴). Conclusion: CM from ADSCs and MenSCs of healthy women enhances apoptosis and inhibits endometriotic MenSCs migration in vitro, suggesting potential therapeutic strategies for endometriosis.

کلیدواژه ها:

Endometriosis ، Mesenchymal stem cells ، Cell migration ، Apoptosis ، Conditioned culture media. ، آندومتریوز ، سلول های بنیادی مزانشیمی ، مهاجرت سلولی ، آپوپتوز ، محیط های کشت شرطی.