Improving morpho-physiological characteristics of camelina (Camelina sativa) by application of salicylic acid and bio-fertilizer under drought stress

In order to improve the morpho-physiological characteristics of Camelina by applying salicylic acid (SA) and biofertilizer under drought stress, a factorial experiment was conducted in a completely randomized design with three replications in the research greenhouse of Yasouj University in ۲۰۲۴. The first factor included drought stress at ۳ levels (irrigation after ۲۰, ۴۰, and ۶۰% moisture depletion), the second factor was the Barvar-۲ phosphate biofertilizer at two levels (application and non-application), and the third factor was foliar spraying of SA at three levels (۰, ۱۰۰, and ۲۰۰ μM). Irrigation at ۴۰% and ۶۰% depletion of available soil moisture significantly reduced plant height, number of lateral branches, leaf chlorophyll index, relative water content, number of seeds per pod, number of seeds per plant, thousand-seed weight and total seed weight per plant, whereas the number of pods per plant increased. Under drought stress, electrolyte leakage and leaf malondialdehyde content were significantly increased, indicating the occurrence of oxidative stress and membrane damage. In contrast, the contents of soluble sugars and proline in leaves increased under water deficit, highlighting the critical role of compatible osmolytes in plant adaptation to drought conditions. The application of Barvar-۲ phosphate biofertilizer and SA increased the studied traits. The highest number of pods per plant (۱۹۳) was obtained from ۶۰% moisture depletion + no application of Barvar-۲ phosphate + ۲۰۰ μM SA and the lowest number (۹۷) was obtained from the treatment combination of ۲۰% moisture depletion + no application of Barvar-۲ phosphate + no application of SA. Also, the highest and lowest total grain weight per plant were obtained from the treatments of ۲۰% moisture depletion + application of Barvar-۲ phosphate + application of ۱۰۰ μM SA and ۶۰% moisture depletion + no application of Barvar-۲ phosphate + no application of SA, respectively. Based on the obtained results, although increasing drought stress reduced the total seed weight per plant, the application of phosphate biofertilizer (Barvar-۲) in combination with salicylic acid (SA), particularly under severe stress levels, was able to partially alleviate the adverse effects of drought on the productive performance of camelina. This positive effect can be attributed to the role of phosphate biofertilizer and SA in enhancing plant growth under stress conditions. Overall, according to the general yield performance criterion, the best treatment was the combination of phosphate biofertilizer (Barvar-۲) with ۱۰۰ μM salicylic acid, which resulted in the highest total seed weight per plant.

کلیدواژه ها:

Camelina ، Drought Stress ، Photosynthetic Pigments ، Barvar-۲ phosphate ، Proline ، کاملینا ، تنش خشکی ، رنگیزه های فتوسنتزی ، فسفات بارور-۲ ، پرولین