The effect of zinc oxide nanoparticles on the physiological characteristics of Lepidium perfoliatum L. under low-temperature stress

The medicinal plant, clasping pepperweed (Lepidium perfoliatum L.), is an annual plant from the Brassicaceae family. Low-temperature stress is one of the important environmental factors limiting plant cultivation. Many compounds have been used to reduce the harmful effects of this stress. In this study, the effect of zinc oxide nanoparticles on the physiological characteristics of the Lepidium perfoliatum L. under low-temperature stress was an experiment conducted as a factorial design in a completely randomized design with three replications. The experimental treatments included temperature at three levels (۲۵ (control), ۵, and -۱°C) and foliar application of zinc oxide nanoparticles at four levels (۰, ۲۵, ۵۰, and ۱۰۰ mg/L). Results showed that in temperature stress conditions, zinc oxide nanoparticle foliar spray increased peroxidase, catalase, and superoxide dismutase enzyme activities immediately and three days after the stress. By decreasing the temperature from ۲۵°C to -۱°C, the non-application of zinc oxide nanoparticles increased electrolyte leakage immediately and three days after the stress. Also, zinc oxide nanoparticle foliar spray in ۱۰۰ mg/l and ۵۰ mg/l reduced the amount of damage to the membrane and electrolyte leakage. At a temperature of -۱°C, zinc oxide nanoparticle foliar spray decreased proline both immediately after and three days after stress. In general, it can be stated that the use of ۱۰۰ mg/l and ۵۰ mg/l zinc oxide nanoparticles had a significant effect on reducing the effects of low-temperature stress in the Lepidium perfoliatum.

کلیدواژه ها:

Antioxidant enzymes ، Cold stress ، Medicinal plant ، Nanoparticles ، Proline ، آنزیم های آنتی اکسیدان ، پرولین ، تنش سرما ، گیاه دارویی ، نانوذرات