The effect of different charcoal levels on physiological traits of sage plant (Salvia officinalis L.) under cadmium stress

Cadmium is one of the heavy metals that has a negative effect on the physiological and metabolic process of the plant. In order to study the effects of different charcoal levels on physiological traits of sage plant (Salvia officinalis L.) under cadmium stress.  One factorial experiment on the basis of completely randomized design with four replications was designed. The experimental treatments included different levels of charcoal (۰, ۱ and ۲% by weight) and cadmium (۰, ۳۰ and ۶۰ mg/kg of soil). The results showed that cadmium cause to decrease photosynthetic pigments and relative water content and increased phenolic compounds, antioxidant activity, electrolyte leakage, soluble carbohydrate and cadmium content. On the other hand, charcoal application in different levels of cadmium cause to improve the studied traits as the treated plants with ۲% w/w charcoal and ۶۰ mg/kg soil cadmium showed the highest total phenol (۱۸۶.۸۷ mg/g FW), total flavonoids (۸۸.۳۲ mg/g FW) and antioxidant activity (۸۰.۴۶%). Also, application of ۲% w/w charcoal at the highest cadmium stress level (۶۰ mg/kg soil) cause to decrease by ۵۸.۸۲% cadmium compared with no charcoal usage at this cadmium level. The highest effect of charcoal on physiological traits was observed at the highest used concentration (۲% w/w) and the plants under the severe cadmium stress. Therefore, correct application of charcoal organic matter is suggested as one of the suitable biological ways to decrease negative effects of cadmium stress in sage plant and other medicinal plant.

کلیدواژه ها:

Antioxidant activity ، Enzyme ، Medicinal plants ، Total phenol ، آنزیم ، فعالیت آنتی اکسیدان ، فنل کل ، گیاهان دارویی