Effects of Fisetin on Cutaneous Full Thickness Wound Healing in a Rat Model

Cutaneous wound healing is a complex biological process involving inflammation, proliferation, and tissue remodeling. Fisetin, a dietary flavonoid found in fruits and vegetables, is known for its potent antioxidant and anti-inflammatory properties. However, its therapeutic potential in wound healing has not been previously explored. This study aimed to evaluate the effects of topical fisetin on skin wound healing in a rat model. Forty male rats were randomly divided into four groups: control (no treatment), olive oil (vehicle), treatment ۱ (T۱; ۱۰ mg/kg fisetin in olive oil), and treatment ۲ (T۲; ۲۰ mg/kg fisetin in olive oil). A full-thickness dorsal wound (۲۰ mm) was created in each rat. Treatments were administered topically for ۱۴ days. Wound contraction was measured on days ۳, ۶, ۹, ۱۲, and ۱۴. Oxidative stress markers malondialdehyde (MDA), Total oxidant status (TOS), superoxide dismutase (SOD), and total antioxidant capacity (TAC) were assessed on day ۱۴, and histological evaluation using H&E staining was performed on days ۷ and ۱۴. Fisetin significantly accelerated wound contraction, particularly in the T۲ group. MDA and TOS levels were significantly reduced, while SOD and TAC were elevated in fisetin-treated groups compared to controls. Histopathological analysis revealed reduced inflammatory cell infiltration, enhanced collagen deposition, and re-epithelialization, especially in the T۲ group. Topical application of fisetin markedly improves cutaneous wound healing by modulating oxidative stress and enhancing tissue regeneration. These findings suggest fisetin as a promising natural agent for promoting skin repair and warrant further investigation in clinical settings.

کلیدواژه ها:

wound healing ، Flavonoids ، oxidative stress ، skin ، Fisetin ، Rat