Impacts of Haematococcus pluvialis extract on the expression of the SOX۲ and OCT۴ genes in the prostate cancer cell line, PC۳

There is a small population of cells with stem cell properties in cancer tissues that are responsible for the initiation of tumour formation and have the ability to resist apoptosis inducing agents and chemotherapy. SOX۲ is a transcription factor in stem cells and OCT۴ is a pluripotency factor known as cancer stem cell markers. The use of plant compounds as cancer suppressors has become increasingly important today. The purpose of this study is to investigate the expression of self-renewal genes SOX۲ and OCT۴ in a prostate cancer cell line (PC۳) treated with different concentrations of methanolic, chloroform, aqueous and hexane extracts of the green microalga Haematococcus pluvialis. PC۳ cells were exposed to varying concentrations of microalgae extract after cultivation. The percentage of survival was checked using the MTT method, and the change in expression change of SOX۲ and OCT۴ genes was studied using the RT-qPCR technique.  The results of the evaluations showed that the hexane extract of microalgae caused a significant decrease in the number of living cells compared to the control and significantly decreased the expression of the SOX۲ and OCT۴ genes. Therefore, the hexane extract of H. pluvialis can be considered a factor affecting the expression pathway in self-renewal genes of stem cells.

کلیدواژه ها:

Prostate cancer ، SOX۲ ، OCT۴ ، Green microalgae ، MTT