Biochemical and molecular identification of nutrient sources on growth of bacteria and bacterial cell Cheshme Khosh reservoirs for Enhanced OilRecovery

Samples of oil from oil reservoirs in order to isolate and identify bacteria Gachsaran completely sterile and the tanks were prepared, Using the public medium and consider the ability to producebio-surfactants, bacteria were evaluated, Were to determine the best conditions for cell growth, carbon sources (whey, soy, starch) added to the specific environment and within 7 days for bacterial growth curve was plotted for each of the bacteria.The best carbon source for growth of bacteria tothe starch and whey and soy. Identification of bacteria using molecular and biochemical methods .Overall, the results of biochemical tests 16sr RNA species in the genus Bacillus, the bacteria were classified as bacteria called Bacillus Megaterium.

کلیدواژه ها:

Microbial Enhanced Oil Recovery ، Megaterium Bacillus bacteria ، microorganisms ، new technologies Recovery