Evaluation of a Newly Established Rapid and Selective Graphene-based Kit for Diagnosis of Hydatidosis by Using Nano ELISA and Nano-immunochromatography

Introduction: Hydatid disease (HD) or hydatidosis is a major public health problem that is spreading all over the world. The diagnosis of hydatid disease is considered to be challenging; however, serological diagnosis provides an early diagnostic approach, and confirmation of the existence of the hydatid cyst microscopically is still necessary. Enzyme-linked immunosorbent assay (ELISA) is one of the best diagnostic methods.Materials & Methods: Sandwich ELISA, nano-sandwich ELISA, and the immunochromatographic test (ICT) were conducted utilizing purified hydatid antigen for the diagnosis of human hydatidosis. The analyzed sera comprised samples from patients infected with hydatidosis (n=۵۰), patients infected with other helminthic parasites (۲۰, ۲۰, and ۱۰ with Schistosoma mansoni, Fasciola hepatica, and Ancylostoma duodenale, respectively), and ۵۰ healthy negative control subjects.   Results: The findings indicated that the sandwich ELISA detected positive results in ۳۹ out of ۵۰ hydatid-infected samples, yielding an assay sensitivity of ۷۸%. Seven out of ۵۰ samples from parasite-infected groups yielded positive results, resulting in ۹۳% specificity. In the nano-sandwich ELISA, ۴۲ of ۵۰ hydatid-infected samples yielded positive results, which produced an assay sensitivity of ۸۴%. seven out of ۵۰ samples from other parasite-infected groups had positive results, giving ۹۳% specificity. Using the ICT, ۳۶ out of ۵۰ hydatid-infected samples yielded positive results, which gave an assay sensitivity of ۷۲%. sixteen out of ۵۰ from other parasite-infected groups had positive results, giving ۸۰% specificity. Diagnostic specificity reached ۹۳%, ۹۳%, and ۸۰% by using sandwich ELISA, nano-sandwich ELISA, and ICT techniques, respectively.    Conclusion: The use of nano-sandwich ELISA is one of the best methods for the diagnosis of human hydatidosis.

کلیدواژه ها:

Ancylostoma ، Diagnostic Kit ، Echinococcosis ، Fasciola ، Schistosoma