Identification of Yersinia Species in Raw Chicken Meat in Tehran Retail Stores and Determination of Their Antibiotic Resistance Pattern

Introduction: Yersinia constitutes one of the predominant bacterial agents implicated in foodborne illnesses. The objective of this investigation is to ascertain the presence of Yersinia species in raw chicken meat procured from retail establishments in Tehran, alongside an examination of their antibiotic resistance patterns.Materials & Methods: Between April and September ۲۰۲۳, a total of ۲۲۰ chicken meat samples were systematically collected and analyzed for contamination by Yersinia species. Initial isolation was conducted through enrichment in saline phosphate at ۴ °C for three weeks, followed by secondary enrichment utilizing ۵.۰% potassium hydroxide. The resultant samples were subsequently cultured on CIN agar medium. Following performing warm staining and the microscopic observation of gram-negative cocci, biochemical assays were employed to differentiate the strains, and the findings were corroborated using the API ۲۰E kit. Ultimately, antibiotic resistance profiles were established via the agar disk diffusion methodology encompassing seven different antibiotics. Results: From ۲۲۰ chicken meat samples, ۱۲(۵.۵%) suspect strains of Yersinia were successfully isolated and definitively identified as Yersinia through biochemical testing. Application of the API ۲۰E kit revealed that the isolates comprised the following species: Y. enterocolitica (۳ strains), Y. intermedia (۵ strains), Y. frederiksenii (۲ strains), and Y. kristensenii (۲ strains). Notably, all isolated strains exhibited resistance to ampicillin, tetracycline, and cefixime, while remaining sensitive to other antibiotics tested. Conclusion: The results of this study indicate the presence of various strains of Yersinia in chicken meat samples across Tehran. Given the emergence of microbial resistance to specific antibiotics, it is imperative that antibiotic usage be managed with judicious strategies.

کلیدواژه ها:

Yersinia ، Yersinia enterocolitica ، Chicken Meat ، Antibiotic resistance