Evaluating the Protective Effect of Melatonin on Apoptosis, Expression of Bax and Bcl۲ Genes, and ROS Level after Radiofrequency Radiation on L۹۲۹ Fibroblast Cell Line

Background: The increasing use of mobile phones has raised concerns about potential health risks. In addition, the effect of protective materials is also important.Objective: This study aimed to evaluate the protective effect of Melatonin on cell viability, apoptosis, Reactive Oxygen Species (ROS) level, and gene expression of Bax and Bcl۲ in L۹۲۹ fibroblast cells after Radiofrequency (RF) radiation.Material and Methods: In this experimental study, cells were divided into six groups with at least ۳ replications in each group. The two groups were respectively exposed to radiofrequency waves for ۸ and ۱۶ hours. They were pretreated with melatonin before radiation exposure, while the control group did not receive any radiation or melatonin during the experiment, and the melatonin group was just treated with melatonin.Results: Cell survival was reduced after ۸ hours of RF radiation. After ۱۶ hours, the total apoptosis was increased, and pretreatment with melatonin increased cell survival and reduced total apoptosis. Radiation increased intracellular ROS levels. RF increased the Bax/Bcl۲ ratio, and the treatment with melatonin decreased it after ۸ hours. Conclusion: Melatonin prevents the increase in apoptosis and Bax/Bcl۲ ratio, but there are no significant changes in survival and ROS levels.

کلیدواژه ها:

Radiofrequency ، Fibroblasts ، L۹۲۹ Cells ، Melatonin ، apoptosis