Inhibitory Effects of Crocin and Crocetin on Platelet Aggregation and Adhesion in Healthy Volunteers

Medicinal plants have shown potential in preventing cardiovascular diseases due to their bioactive compounds. This study evaluated the inhibitory effects of saffron-derived crocin and crocetin on platelet aggregation induced by various agonists and also their impact on platelet adhesion. This experimental laboratory study included ۱۰ healthy individuals. Hemostasis was assessed using prothrombin time (PT) and partial thromboplastin time (PTT) tests, while complete blood count (CBC) analysis was performed with a Sysmex hematology analyzer. Platelet aggregation was evaluated in the presence of crocin and crocetin using a Chronolog dual-channel aggregometer with agonists including collagen, ADP, epinephrine, and calcium ionophore A۲۳۱۸۷. Platelet adhesion was also assessed using the Bellavite method and ELISA. Statistical analyses were performed with SPSS version ۲۴ and GraphPad Prism version ۸.  A p-value < ۰.۰۵ indicated statistical significance. The participants had an average age of ۲۷.۳ ± ۳.۴ years. PT, PTT, and CBC indices were within normal ranges. Both crocin and crocetin significantly inhibited platelet aggregation in a dose-dependent manner compared to the untreated group (p < ۰.۰۰۱). Platelet adhesion also decreased considerably at higher concentrations of crocin and crocetin (۱۰۰ and ۲۰۰ µg/mL) compared to the control group (p < ۰.۰۱). Crocin and crocetin, key bioactive compounds in saffron, demonstrated potent antiplatelet effects by inhibiting platelet aggregation and adhesion. These findings suggest that these compounds could prevent and potentially treat thrombotic disorders.

کلیدواژه ها:

Crocin ، crocetin ، Platelet Aggregation ، Platelet Adhesion