Dexmedetomidine protects against ischemia and reperfusion-induced kidney injury in rats by inhibiting the expression of TRPM۲ and TRPA۱ channels

Objective(s): Renal ischemia and reperfusion (I/R) injury is a potentially serious issue encountered during various medical and surgical procedures. This condition is clinically significant because of its high incidence and mortality rate. In this study, we aimed to investigate the protective effect of dexmedetomidine (DEX) on oxidative stress-activated TRPM۲ and TRPA۱ channel expression in rats with a renal I/R model.Materials and Methods: A total of ۳۵ rats were used in the study. The animals were divided into five groups. The control group received no procedure during the experiment. In the sham group, the abdomen was opened under general anesthesia, the right kidney was removed, and the left renal pedicle was exposed; however, a renal clamp was not applied. The dexmedetomidine group received dexmedetomidine, using the same surgical procedure. In the I/R groups, rats were subjected to left renal ischemia for ۳۰ min followed by ۴۵ min of reperfusion. Dexmedetomidine was not administered to the I/R group, while dexmedetomidine was given to the I/R+DEX group at the beginning of reperfusion. TRPM۲ and TRPA۱ levels were analysed in serum and kidney tissues at the end of the experiment.Results: A significant increase in TRPM۲ and TRPA۱ expression was observed in all samples from the I/R group compared to the non-I/R groups, while a significant decrease in TRPM۲ and TRPA۱ expression was seen at the I/R+DEX compared to the I/R.Conclusion: Dexmedetomidine may have renoprotective effects in I/R injury by inhibiting redox-TRP channels.

کلیدواژه ها:

Dexmedetomidine ، Ischemia/Reperfusion ، Kidney ، TRPA۱ ، TRPM۲