Dual-Functional Orthopedic Implants based on Al۲O۳-CuO/Ti nanocomposite: Antimicrobial and Osteogenic Properties

In this study, we investigated the antimicrobial, bioactivity, and in vitro cytotoxicity of a nanocomposite made of copper oxide (CuO) and aluminum oxide (Al۲O۳) with two different morphologies of copper oxide (Spherical-sCuO and Nanoplate-pCuO), which was made using the Spark Plasma Sintering (SPS) process on a titanium substrate as an orthopedic implant. Two different weight percents of copper oxide nanostructures of sCuO NP (۱۰ wt%, ۲۰ wt%) and pCuO NP (۱۰ wt%, ۲۰ wt%) have been used in this research. Synthesized nanocomposites were investigated by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), and field emission scanning electron microscope (FESEM). Based on the obtained results, the XRD pattern and XPS confirmed that the nanocomposites were successfully synthesized without impurity. FESEM images showed that CuO nanoparticles and nanoplates were distributed on the alumina matrix homogeneously. The antibacterial activity of synthesized nanocomposites was investigated using Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), gram-negative and gram-positive bacteria, respectively. Antibacterial activity results showed that CuO nanoparticles had high antibacterial activity, and the effect of CuO nanostuctures depended not only on their morphology and size, but also on the type of microorganisms. Furthermore nanocomposite with nanoplate copper oxide exhibited more bioactivity properties than the spherical shape. S. aureus showed greater resistance to CuO nanostructure, while E. coli was more susceptible to them (۱۵%). In addition, toxicity tests showed that nanoplate copper oxide exhibited greater toxicity due to its high surface reactivity than spherical nanoparticles. This study provides new insights into the role of copper oxide nanoparticle morphology in the properties of nanocomposites for use as orthopedic implants.

کلیدواژه ها:

Nanocomposite ، CuO ، Alumina ، SPS ، Antibacterial Properties