Evaluation of Egg Drop Syndrome Virus Fiber Protein as a Vaccine Candidate: In Silico Analysis, Expression, Purification and Its Stability

The Egg Drop Syndrome Virus (EDSV), an avian adenovirus, triggers a sharp decline in both egg production and quality in infected chickens, leading to substantial economic losses in poultry industry. Previous studies have suggested that the EDSV fiber protein may serve as a candidate for subunit vaccine. The present research focused on the expression, purification, and thermal stability evaluation of recombinant fiber protein as a vaccine against EDSV. Using an in silico approach, we investigated the fiber protein structure and its expression in Escherichia coli (E. coli). We also evaluated the thermal stability of the expressed protein. The protein was expressed predominantly as soluble trimeric proteins in E. coli and purified using nickel-affinity purification method, yielding approximately ۱۵ mg/L of purified protein. Structural analysis using immunological and bioinformatics tools Confirmed retention of the native trimeric conformation in the recombinant protein. Based on the thermal stability evaluation on this recombinant protein, the protein showed good thermal stability, highlighting its potential as a subunit vaccine candidate for a vaccine against EDSV.

کلیدواژه ها:

Egg drops syndrome virus ، Fiber protein ، In silico ، purification ، vaccines