Effect of Microencapsulated Saccharomyces Boulardii on IgA Secretion from Intestinal Epithelia in Wistar Rats

Background: Probiotics are live microorganisms that confer health benefits to their host. The intestinal microbiota represent the largest source of microbial variation and play a significant role in host responses in health and disease. This study was designed to evaluate the effect of administering capsulated Saccharomyces species on gastrointestinal tract properties in rats. Methods: Male Wistar rats weighing ۱۰۰-۱۱۰g were divided into three groups, each containing ۱۰ rats: a control group receiving normal saline, a group treated with uncapsulated Saccharomyces boulardii (T group), and a group treated with microencapsulated S. boulardii (MT group). Probiotics were administered at a dosage of ۲g/kg for ۸ continuous weeks. IgA was measured in duodenal and jejunal washings using an ELISA assay according to the manufacturer’s instructions. Results: Administration of S. boulardii significantly increased IgA secretion from the duodenum and jejunum compared to the control group (P<۰.۰۵). The increase was notably higher in the MT group, with mean IgA levels of ۴۶۰±۳.۴۷ µg/mL in the duodenum and ۵۴۰.۳±۲.۱۴ µg/mL in the jejunum, compared to ۳۴۰.۷±۰.۹۲ µg/mL and ۲۴۲±۰.۵۷ µg/mL in the T group, respectively. Conclusion: The results indicate that S. boulardii, particularly in microencapsulated form, enhances IgA secretion, suggesting its potential as a probiotic yeast with immunostimulatory effects for the treatment of gastrointestinal disorders.

کلیدواژه ها:

Probiotic ، IgA ، Intestine ، Saccharomyces Boulardii