Comparison of wound healing abilities of two nanocomposites containing ZnO/magnetite and ZnO/Malachite on infected excisional wound healing

Background: Delays in infected wound healing are usually caused by bacterial infection and local inflammation, imposing a significant and often underappreciated burden on patients and society. In this study, the effects of two nanocomposites, ZnO/magnetite (ZnMng-NCT) and ZnO/Malachite (ZnMch-NCT), which possess antibacterial and antioxidant properties and have been widely used for various therapeutic purposes, were compared in terms of their ability to enhance wound healing. This study aimed to evaluate the effects of topical application of ointments prepared from ZnMag-NCT and ZnMch-NCT on excisional infected wound healing in a mouse model. Methods: ۳۶ healthy male mice were used in this experiment. Using surgical techniques, a single full-thickness wound (۵ mm in diameter) was created on the dorsal surface of each mouse. An aliquot containing ۲ × ۱۰⁷ CFU of Staphylococcus aureus (StAu) and Pseudomonas aeruginosa (PsAe) was inoculated into the wound. The animals were then divided into four groups: control, mupirocin® ointment, and two treatment groups receiving ۲% ZnMag-NCT and ZnMch-NCT ointments, respectively. To evaluate the effects of ointments, in vitro antibacterial activity and in vivo wound size were measured. Result: MIC and MBC values for ZnMch-NCT were ۶.۰۵ ± ۰.۶۶ μg/mL and ۵.۷۵ ± ۰.۴۵ μg/mL for StAu, and ۵.۹۵ ± ۰.۷۳ μg/mL and ۵.۸۰ ± ۰.۶۵ μg/mL for PsAe. For ZnMag-NCT, these values were ۶.۹۰ ± ۰.۵۰ μg/mL and ۶.۰۰ ± ۰.۹۲ μg/mL for StAu, and ۶.۲۰ ± ۰.۹۰ μg/mL and ۶.۱۰ ± ۰.۹۹ μg/mL for PsAe. Topical application of ZnMag-NCT and ZnMch-NCT significantly (p < ۰.۰۵) decreased wound area and total tissue bacterial count. Conclusion: In summary, it can be concluded that ZnMch-NCT nanocomposite can accelerate the healing of infected wounds through its antibacterial properties. Future studies should investigate long-term degradation, immune responses, and the scalability of this treatment for clinical applications.

کلیدواژه ها:

Infected wound healing ، nanocomposite ، ZnO/magnetite ، ZnO/Malachite