In situ chemosterilization in mature male golden hamsters using white onion juice-derived carbon quantum dots: a proof of concept spermatological study

Background: In situ non-invasive chemical castration has been suggested as an alternative to surgical method regarding minimal post-operative complications and costs. This study was chalked out to pin down the effect of intra-testicular injection of white onion (Allium cepa) juice-derived carbon quantum dots (CQDs) on spermatological parameters of mature golden hamsters (Mesocricetus auratus). Methods: Fifteen mature male golden hamsters of similar age and weight were randomly assigned into three equal groups, including control, sham, and CQDs (۴۰.۰۰ mg/kg into each testicle) groups. The CQDs were synthesized through one-step hydrothermal carbonization method and the experimental period was ۳۵ days. Spermatological analysis included epididymal sperm concentration, total and progressive motilities, and motility characteristics, as well as plasma membrane functionality, DNA damage, viability, and abnormal morphology determinations. Results: Intra-testicular injection of CQDs led to pronounced reductions in sperm kinematic parameters, plasma membrane functionality, and viability along with marked rises in DNA damage and morphological abnormality. Conclusion: The CQDs might be the promising chemical sterilizing agents in mature male golden hamsters; however, further research is warranted to divulge their long-lasting efficiency and/or possible systemic side effects.

کلیدواژه ها:

Carbon quantum dots ، Golden hamster ، Non-surgical sterilization ، Sperm