Background: Campylobacter species are Gram-negative, motile, and relatively slow-growing bacteria that colonize in the intestinal and genital tracts of a wide range of domestic animals. Certain pathogenic strains can induce systemic infections, particularly in susceptible pregnant ewes, where the infection manifests as bacteremia followed by placentitis, fetal infection, and abortion. Historically, C. fetus subsp. fetus has been the predominant species linked to ovine abortion globally. However, recent epidemiological studies have identified an increasing prevalence of C. jejuni as a significant causative agent in certain regions. This study aims to perform molecular identification and isolation of C. jejuni strains associated with sheep abortion. Methods: Collected samples for the bacteriological examination and DNA extraction included abomasal contents, lung, liver, heart, kidney and spleen. The specimens were cultured on blood agar and MacConkey agar and incubated at ۳۷ °C with ۱۰% CO۲. DNA was extracted using CinnaGen commercial kit specified for bacterial DNA extraction from tissues. Conventional polymerase chain reaction (PCR) was performed in accordance with the protocol described by Ilhan et al. (۲۰۲۱) to amplify ۹۷۷ bp and ۳۴۴ bp fragments for the detection of C.fetus subsp. fetus and C. jejuni, respectively. Results: White, spreading, smooth colonies on blood agar and non-lactose fermentative colonies on MacConkey agar were isolated. The results of Gram staining method on the isolated colonies indicated Gram-negative spiral-shaped bacteria. The molecular analysis which was conducted on both tissue samples and the colonies, revealed C. jejuni in abomasal contents and the pure colonies. Conclusion: Based on the culture and molecular results, C. jejuni has been confirmed as a causative agent of abortion in the studied lamb, warranting significant attention comparable to that of C. fetus, which has traditionally been the primary suspect in such cases. The rising prevalence of C. jejuni infections underscores the need for enhanced biosecurity measures. These include routine vaccination, strict farm hygiene protocols (such as wearing gloves during lambing, prompt removal of abortion materials, and isolation of affected ewes), regular disinfection of pens and feed troughs, and minimizing bird populations in livestock housing to mitigate transmission risks.