In-Vitro Evaluation of Antimicrobial Efficacy of Spherical Green Ag-Tio۲ NPs Using Sajne Leaves Extricate of Moringa Oleifera in Aqueous Media

Environmentally friendly and biocompatible methods were employed for the green synthesis of Ag-TiO۲ nanoparticles (NPs) using the leaf extract of Moringa oleifera as a capping and reducing agent. The successful synthesis of Ag-TiO۲ NPs was observed through a wavelength shift within one hour of reaction. The formation of nanoparticles was confirmed through multiple analytical techniques, including UV–VIS spectroscopy, FTIR, XRD, EDX, and SEM analysis. X-ray diffraction and electron microscopy confirmed the spherical structure and size of the nanoparticles. EDX analysis verified the presence of silver ions in the doped TiO۲ lattice, while SEM revealed the spherical morphology of Ag-TiO۲ NPs. Bacterial concentrations of ۱.۸ × ۱۰⁸ CFU/ml were tested using ۲۰ ml of three samples: Moringa oleifera leaf extract, TiO۲ NPs, and Ag-TiO۲ NPs at concentrations of ۱۲.۵ mg/L, ۲۵ mg/L, and ۵۰ mg/L, in three separate trials. The nanoparticles demonstrated significant antimicrobial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli), as well as antifungal activity against Aspergillus niger (A. niger). These findings suggest that Ag-TiO۲ NPs hold potential for applications in biomedical fields.Environmentally friendly and biocompatible methods were employed for the green synthesis of Ag-TiO۲ nanoparticles (NPs) using the leaf extract of Moringa oleifera as a capping and reducing agent. The successful synthesis of Ag-TiO۲ NPs was observed through a wavelength shift within one hour of reaction. The formation of nanoparticles was confirmed through multiple analytical techniques, including UV–VIS spectroscopy, FTIR, XRD, EDX, and SEM analysis. X-ray diffraction and electron microscopy confirmed the spherical structure and size of the nanoparticles. EDX analysis verified the presence of silver ions in the doped TiO۲ lattice, while SEM revealed the spherical morphology of Ag-TiO۲ NPs. Bacterial concentrations of ۱.۸ × ۱۰⁸ CFU/ml were tested using ۲۰ ml of three samples: Moringa oleifera leaf extract, TiO۲ NPs, and Ag-TiO۲ NPs at concentrations of ۱۲.۵ mg/L, ۲۵ mg/L, and ۵۰ mg/L, in three separate trials. The nanoparticles demonstrated significant antimicrobial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli), as well as antifungal activity against Aspergillus niger (A. niger). These findings suggest that Ag-TiO۲ NPs hold potential for applications in biomedical fields.

کلیدواژه ها:

Green synthesis ، NPs ، Moringa oleifera ، Antibacterial activity ، Antifungal activity