Purification and Characterization of a Novel Thermostable and Acid Stable α-Amylase from Bacillus Sp. Iranian S1
محل انتشار: ماهنامه بین المللی مهندسی، دوره: 26، شماره: 8
سال انتشار: 1392
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 711
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شناسه ملی سند علمی:
JR_IJE-26-8_007
تاریخ نمایه سازی: 17 خرداد 1393
چکیده مقاله:
This study describes the purification and biochemical characterization of a novel thermostable and acidic pH stable α -amylase from Bacillus sp.Iranian S1 which isolated from the desert soil (Gandom-e-Beryan in Lut desert, Iran). Maximum enzyme production was in exponential phase with activity 2.93 Uml-1 at 50°C and pH 5. The enzyme was purified by isopropanol sedimentation, ion-exchange chromatography on DEAE cellulose DE-52 and gel filtration on Sephadex G-100. The purification procedure performed for α-amylase enzyme achieved about 98.9-fold purification with 25% yield andspecific activity 375.9 U mg-1 of protein. The molecular weight of purified α-amylase was estimated around 70 KDa by SDS-PAGE and it was stable in a wide range of temperature and pH between 30°C- 110°C and 3-9, respectively. Assayed with soluble starch as substrate, the enzyme displayed optimalactivity at 90°C and pH 5. The purified α-amylase was acid- and thermo-stable with novel properties making it suitable for many industrial purposes
کلیدواژه ها:
نویسندگان
s Sharifi Alghabpoor
Department of Microbiology, Plant and Microbe Biotechnology, Yerevan State University, A. Manoogian ۱, ۰۰۲۵, Yerevan, Armenia
h Panosyan
Department of Microbiology, Plant and Microbe Biotechnology, Yerevan State University, A. Manoogian ۱, ۰۰۲۵, Yerevan, Armenia
a Trchounian
Department of Microbiology, Plant and Microbe Biotechnology, Yerevan State University, A. Manoogian ۱, ۰۰۲۵, Yerevan, Armenia
y Popov
Department of Microbiology, Plant and Microbe Biotechnology, Yerevan State University, A. Manoogian ۱, ۰۰۲۵, Yerevan, Armenia