Structural and Functional Exploration of UTS۲B Gene for a Possible Role in Poultry Ascites Syndrome: In Silico Analysis

Ascites syndrome is a multifactorial disease in poultry, associated with high mortality and significant economic losses. Genomic studies have identified UTS۲B gene as a candidate gene associated with ascites syndrome in poultry. Therefore, this study employed an in-silico approach to explore the structural and functional characteristics of the UTS۲B protein and its potential link to ascites. The amino-acid sequence of UTS۲B protein was retrieved from the NCBI database and subjected to analyze through ProtParam, ProtScale, NetPhos, NetNGlyc, LocTree۳, and SignalP tools to determine its sequence features and physicochemical properties. Secondary and tertiary structures were predicted using I-TASSER, AlphaFold, and trRosetta, with structural quality validated by PROCHECK (Ramachandran plot analysis) and ERRAT scores. Promoter analysis was performed using MEME Suite, TOMTOM, and GOMo to identify regulatory motifs. The UTS۲B protein exhibited a theoretical isoelectric point of ۵.۶۷, an instability index of ۴۲.۳۳, and an average hydrophilicity coefficient of -۰.۱۹۹, indicating a hydrophilic and moderately unstable nature. It contains ۱۷ phosphorylation sites and ۴ N-glycosylation sites, suggesting post-translational modifications that impact extracellular activity in peptide signalling pathways. Secondary structure analysis revealed a predominance of alpha helices and random loops. Among the predicted ۳D models, the trRosetta structure achieved the highest quality, with ۹۲.۴% of residues in favorable regions of the Ramachandran plot and an ERRAT score of ۸۵.۶. Protein network analysis indicated that UTS۲B may interact with proteins involved in pathways associated with calcium ion binding and cellular stress responses, both of which play a critical role in the pathophysiology of ascites. Promoter analysis identified regulatory motifs potentially linked to ion transport and inflammation, providing further insights into gene regulation. The findings suggest that UTS۲B may play a role in calcium ion signalling and peptide-mediated extracellular pathways, contributing to ascites syndrome. This study presented critical bioinformatics insights into the UTS۲B protein, providing foundational knowledge for future research aimed at understanding its role in ascites syndrome.

کلیدواژه ها:

Ascites syndrome ، Poultry ، Bioinformatics ، UTS۲B gene ، Tertiary structure