Antioxidant, biochemical, hematological indices and handling stress resistance in rainbow trout (Oncorhynchus mykiss) fed with diets supplemented with nano-selenium and vitamin C

Use of various nanoparticles and vitamins to strengthen the immune system of fish to improve growth and biochemical indice, and to mitigate the harmful effects of free radicals through enhanced antioxidant enzyme activity has become the subject of numerous studies. In this study, fish with an average weight of ۵۵.۲۰ ± ۷.۹۰ g, after a two-week acclimation period, were randomly distributed into ۱۸ polyethylene ۳۰۰-L tanks, with ۲۵ fish per tank. The experiment consisted of six treatments with three replicates: Control, vitamin (Vit) C۱۰۰, nano-selenium (N-Se)۰.۴۰, Vit C۱۰۰ + N-Se۰.۱۰, Vit C۲۰۰ + N-Se۰.۲۰, and Vit C۴۰۰ + N-Se۰.۴۰. Blood samples were collected post-treatment for analysis of antioxidant enzyme activities, biochemical and hematological indices as well as post-handling stress. Results indicated significant differences in antioxidant enzyme activities among treatments. The highest catalase activity was observed in Vit C۱۰۰ and Vit C۱۰۰ + N-Se۰.۱۰ groups. Glutathione peroxidase activity was significantly higher in the control and Vit C۲۰۰ + N-Se۰.۲۰ groups. Malondialdehyde was the highest in Vit C۱۰۰ + N-Se۰.۱۰ group. Superoxide dismutase activity peaked in Vit C۲۰۰ + N-Se۰.۲۰ group. Biochemical indices showed significant differences among treatments except for high-density lipoprotein. Hemoglobin and red blood cell counts were significantly different. The Vit C۱۰۰ + N-Se۰.۱۰ group showed the highest serum cortisol and glucose levels post-handling stress. Overall, dietary N-Se and Vit C supplementation improved antioxidant, biochemical, hematological indices and stress resistance in rainbow trout.

کلیدواژه ها:

Antioxidant enzyme ، Biochemical indices ، nanoparticles ، Rainbow trout