Polymorphism of pyruvate carboxylase gene and its relation with productive and reproductive performance of dairy cows

Genetic selection in dairy cattle can be enhanced by identifying the candidate genes and variants associated with economic traits. This study examined the productive and reproductive characteristics of Holstein cattle based on the genotypes of the pyruvate carboxylase (PC) gene. A total of ۵۰ cows, aged three to five years, was included in the study, with identical feeding and maintenance protocols applied to all animals. All cows completed a lactation period of ۳۰۵ days. The genotype and allele frequencies were determined through genotyping. Sequencing analysis of ۸۱۶ bp amplicons detected two single-nucleotide polymorphisms (SNPs), rs۴۲۱۹۵۰۰۳ and rs۱۱۰۴۷۶۷۷۶. The association analysis of the two SNPs was not significantly associated with lactation period, dry period, milk composition, and reproductive performance (P>۰.۰۵). In contrast, the highest milk production (۴۳۰۵.۶۳±۲۲۲.۰۱ kg), was associated with the CT genotype of the PC (rs۱۱۰۴۷۶۷۷۶) polymorphism. The results revealed that the SNP rs۱۱۰۴۷۶۷۷۶ in the PC gene was associated with total milk production in Holstein cows. This study may provide valuable insights into the role of the analyzed gene in the functional traits of dairy cattle; however, it is essential to validate the results by researching larger populations and different breeds of cattle.

کلیدواژه ها:

Cattle ، milk traits ، pyruvate carboxylase ، Polymorphisms ، reproductive traits