Selenium-Curcumin-PEG Nanoparticles Radiosensitization for Intensity-Modulated Radiation Therapy of Lung Tumor Cells: In Vitro Synergistic Combination Therapy

Background: Lung cancer is a leading cause of cancer-related mortality worldwide, underscoring the need for the development of more effective treatment strategies. Radiotherapy (RT), particularly intensity-modulated radiation therapy (IMRT), has enhanced tumor targeting while minimizing damage to healthy tissues. Nevertheless, radioresistance and challenges posed by the tumor microenvironment limit its efficacy.Objective: Selenium-curcumin-polyethylene glycol ۶۰۰ nanoparticles (Se-Cur-PEG NPs) analyzed as radiosensitizers in IMRT for lung cancer treatment.Material and Methods: In this experimental study, Se-Cur-PEG NPs were synthesized and characterized for their potential as radiosensitizers.Results: The in vitro toxicity of Se-Cur-PEG NPs against A۵۴۹ lung cancer cells was evaluated using MTT assays, demonstrating a dose-dependent reduction in cell viability. The combination of Se-Cur-PEG NPs (۵۰ µg mL-۱) with IMRT (۴ Gy) resulted in a significant enhancement in cell death compared to either treatment alone, indicating a strong synergistic effect (CI=۱.۲۱) and a notable sensitizer enhancement ratio (SER=۲.۵). Intracellular ROS generation analysis confirmed that Se-Cur-PEG NPs amplified IMRT-induced oxidative stress, contributing to increased cancer cell toxicity. Conclusion: These findings suggest that Se-Cur-PEG NPs hold promise as effective radiosensitizers, potentially improving lung cancer RT outcomes.

کلیدواژه ها:

Intensity-modulated ، Lung Neoplasms ، Selenium nanoparticles ، Oxidative stress ، Radiation-sensitizing agents